WebTMR biocytin is a cell-impermeant, fixable polar tracer that combines the tetramethylrhodamine (TAMRA) with biotin and an aldehyde-fixable primary amine. Polar tracers are commonly used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during … WebBiocytin is invaluable as a rapidly transporting, sensitive neuronal tracer requiring little permeabilizing agents. It is an especially versatile marker for neuroanatomical …

Molecular Probes™ Biocytin (ε-Biotinoyl-L-Lysine) - Fisher Sci

WebDiazo derivatives of biotin, e.g., diazobenzoyl biocytin (DBB), selectively modify both tyrosines and histidines in proteins (16). Unfortunately, the diazo group is unstable during … WebBiocytin Control, 1 mM, 100 μl . 20X Phosphate Buffered Saline, 3 ml . Storage: Upon receipt store at 4°C. Product shipped with an ice pack. Introduction . The Thermo … ipod clickwheel reset

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WebBiocytin hydrazide is an aldehyde-fixable cellular tracer that can be introduced into cells by microinjection. ... Storage conditions can be found on the product information sheet or … WebBiotinidase cleaves biotin from biocytin and, thus, provides the cofactor for the biotin-dependent carboxylases, which are involved in fatty acid synthesis, amino acid catabolism, and gluconeogenesis. Hence, its deficiency results in multiple carboxylase deficiency and secondary ketoacidosis, hyperammonemia, and organic aciduria. WebStorage: Upon receipt store product at room temperature. Product shipped at ambient temperature. Introduction Hydrazide-biotin reagents such as Thermo Scientific™ EZ-Link™ Hydrazide Biocytin are useful for biotinylating macromolecules at carbohydrate groups that have been oxidized to form aldehydes. The hydrazide group reacts with orbis lone worker device instructions