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WebMar 5, 2024 · Incubate at 37 C until cells have detached. Observe cell monolayer using an inverted microscope to ensure complete cell detachment from the surface of the flask. Gently tap flask to dislodge cells. 6. Add 5 10 mL of complete medium to the flask. Tilt the flask in all directions to thoroughly rinse the flask. http://www.utopbio.cn/archives/date/2024/04/page/8 openmee software
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