WebOct 29, 2010 · Fragments of the genes encoding internal transcribed spacers (ITS), intergenic spacers (IGS) and β-tubulin genes were amplified from each isolate by PCR using primer pairs ITS1/ITS4, NF2/NF4 and Fu-tubulin3/FSB2 and the conditions described by White et al., Chen et al., respectively. PCR products were first purified using the PCR …
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