WebFeb 15, 2024 · iRFP670 is a dimeric infra-red fluorescent protein with an excitation peak of 643nm and an emission peak of 670nm in vitro. It is an artificial derivative of the bacteriophytochrome encoded by the Rhodopseudomonas palustris RpBphP6 gene (PMID:23770755).In order to fluoresce, iRFP670 requires a biliverdin (linear tetrapyrrole) … WebJan 1, 2024 · We chose mTagBFP2 (blue), mNeonGreen (green), mApple (red), and iRFP670 (near Infrared) for which the spectra match with the classic excitation laser lines (405 nm, 488 nm, 561 nm, 640 nm) and CHROMA® Quad bandpass filter 89100bs, so that we can conduct fast 4-color imaging without switching between filters and minimizing crosstalk …
(PDF) Two-Color Imaging using Spectral Variants of …
WebiRFP670 1 .. 936 = 936 bp 311 amino acids = 34.5 kDa Product: near-infrared fluorescent protein with an emission peak at 670 nm, engineered from a bacterial phytochrome (Shcherbakova and Verkhusha, 2013) derived from Rhodopseudomonas palustris RpBphP6 iRFP670 1 .. 936 = 936 bp 311 amino acids = 34.5 kDa WebNov 19, 2015 · Interestingly, blue-shifted iRFP670 and iRFP682 have higher quantum yields than their respective red-shifted NIR FP variants. Here we developed a novel NIR FP, named BphP1-FP, from wild-type Rp BphP1. BphP1-FP exhibits the most blue-shifted absorbance and fluorescence emission and the highest quantum yield among the available BphP … portalvision advanced imaging 使用说明
Decay-associated spectra (DAS) of picosecond time
WebFeb 22, 2024 · miRFP670-2 is a basic (constitutively fluorescent) near ir fluorescent … WebFluorescence spectra for five purified iRFP proteins. Fluorescence excitation (a) and emission (b) spectra are shown for iRFP670, iRFP682, iRFP702, iRFP713 and iRFP720 fluorescent proteins.... WebJan 4, 2016 · We studied spectral properties of the iRFP670, iRFP682 and iRFP713 proteins and their mutants having Cys residues able to bind BV either in both PAS (Cys15) and GAF (Cys256) domains, in one of these domains, or without these Cys residues. portalverbund online-gateway