Phospho-akt thr308
WebThe Phospho-Akt (Thr308) Whole Cell Lysate Kit provides assay-specific components for the quantitative determination of phospho-Akt (Ser308) and total Akt in human, mouse, and rat whole cell lysates. Specifications Kit Contents Documentation References WebDec 31, 2009 · Protein were transferred to PVDF membrane and immunoblotted with anti-phospho-Akt(ser473), antiphospho-Akt(thr308), anti-phospho-Erk1/2, anti-phospho-GSK3β (Cell Signaling Technology, Beverly, MA), anti-ChoKα, anti-HSP60, anti-tubulin, anti-GAPDH. Image J program was used to quantified the western blot signal. Phospho-Akt(ser473) …
Phospho-akt thr308
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WebNov 7, 2015 · Antibodies against total phospho-AMPK(Thr172), total phospho-ACC(Ser79), total phospho-p53(Ser15), phospho-Raptor (Ser722/ Ser792), total phospho-mTOR(Ser2448), total phospho-p70S6K(Thr389), total phospho4EBP-1 (Thr 37/46), total phospho-Akt(Ser 473), LC3 I/II β-actinwere obtained fromCell Signaling Technology, Danvers, MA. mTOR … WebAntibodies and Reagents —Antibodies employed include the following: anti-phospho-Akt Ser-473, anti-phospho-Akt Thr-308, anti-phospho-ERK1/2 (Thr-202/Tyr-204), anti-HA, pan-anti-phospho- (Ser/Thr) Akt substrate, anti-phospho-FoxO1/3a (Thr-24/Thr-32), and anti-PP2A/B from Cell Signaling; and anti-PP2A/A from Santa Cruz Biotechnology; anti-PP2A/B …
WebImmunofluorescent analysis of Phospho-AKT pThr308 in serum-starved HeLa cells treated with insulin (100 ng/mL, 15 min) using a Phospho-AKT pThr308 recombinant rabbit monoclonal antibody ( Product # 701052) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). WebAkt/PKB growth Ser241 Thr308 Ser473 Akt/PKB Thr308 Ser473 glycogen synthesis proliferation survival PI3K Akt, Akt Section 03-02-06 PIP 3 PIP 3 Tyr376 Tyr373 SHIP 05_PW_Akt/PKB.eps Tyr326 Src M e m b r a n e PDK1 P P P P P P P P P P P P P P P P mTOR SIN1 GβL Rictor Akt-PKB KSignaling Pathway Phospho-Akt (Ser473) kDa 105 76 57 46.5 ...
Webthese cells. Silencing of B55 phatase (PHLPP) (17) was reported to regulate Akt activity.expression markedly increased phosphorylation at Thr-308 but not at Ser-473 in both FL5.12 cells and NIH3T3 cells. Consistently, PP2A AB55 C holoen-zymes preferentially dephosphorylated phospho-Thr-308 rather than phospho-Ser-473 in in vitro ... WebMar 3, 2024 · Phospho-Akt (p-Akt) on Ser473 (S473), p-Akt on Thr308 (T308), and Akt protein expression in cancerous tissues and survival analysis of ovarian cancer patients were directly obtained from The Cancer Proteome Atlas (TCPA) database 1 …
WebThis Anti-phospho-Akt (Thr308) Antibody is validated for use in IH (P) for the detection of phospho-Akt (Thr308). Anti-phospho-Akt (Thr308) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. SDS CoA
WebPhospho-AKT (Thr308) Monoclonal Antibody (18F3.H11) (200-301-269) Popular TaqMan Real-Time PCR Assays Antibodies Oligos, Primers & Probes GeneArt Gene Synthesis Cell Culture Plastics Applications & … cysto with stent removalWebAberrant PI3K/Akt/mTOR signaling activation is often associated with enhanced Akt phosphorylation, mediated by phosphorylation at S473 by PDK1 and T308 by mTORC2. The OS of AML patients presenting ... binding snowboard women\\u0027s aggressiveWebHK2 isoform is under physiological conditions predominantly localized in the cytosol and upon stimulation of PI3K/ Akt pathway associates with mitochondria and thus can prevent apoptosis. The purpose of this study was to investigate expression and subcellular localization of both HK isoforms in left (LV) and right (RV) heart ventricles of adult ... cysto with turbt cpt codeWebMore>> Detect phospho-Akt (Thr308) using this Anti-phospho-Akt (Thr308) Antibody, clone 50-1C-25 validated for use in WB, IF, IC, ELISA. Less<< MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. bindings of lightning reflexes tbc patternWebInvitrogen Anti-Phospho-AKT1 (Thr308) Polyclonal, Catalog # 44-602G. Tested in Western Blot (WB), Immunohistochemistry (Paraffin) (IHC (P)) and Flow Cytometry (Flow) applications. This antibody reacts with Human, … bindings of raging tempestsWebAKT phosphorylation sites of Ser473 and Thr308 regulate AKT degradation. Protein kinase B (AKT) is a serine-threonine kinase that mediates diverse cellular processes in a variety … cysto with urethrotomyWebPhospho (Thr308, Thr309, Thr305) View Product On Supplier's Website Request a Quote from Cell Signaling Technology Supplier provided information Immunogen Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr308 of mouse Akt. cysto with stone extraction cpt