Ribofecttm
Webbperformed using riboFECTTM CP (RiboBio, Guangzhou, China) with reference to the manufacturer’s recommended protocol. Cell proliferation assay The Cell Counting Kit-8 assay (Sigma-Aldrich, St. Louis, MO, USA) was performed to assay cell proliferation. WebbSmall RNA转染. Long RNA转染. 转染指示剂. 蛋白或多肽转染. 细胞转染是外源基因进入真核细胞的一种重要方法。. 这些外源DNA或RNA通过转染试剂的包装辅助，才能顺利进入细 …
Ribofecttm
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WebbsiRNA (siCtrl) and riboFECTTM CP Transfection kit were purchased from RiboBio Co., Ltd. (Guangzhou, China). Cellular ROS Detection Reagent (CM-H2DCFDA) was purchased from Invitrogen (Thermo Fisher Scientific Inc., Waltham, MA, US). Mouse IL-1β ELISA Kit was purchased from MultiSciences(Lianke)Biotech Co., Ltd. (Hangzhou, China). Webbtransfected with riboFECTTM CP Reagent accord-ing to the manufacturer’s protocol. Bronchial epithelial cells WT mice were transiently transfected with 20 nM scramble or siRNA. After transfection for 24 h at 37 °C in 5% CO 2, the cells were prepared as IL-17A group, CSE group, CSE+ IL-17A group and controls as described
Webb10 feb. 2024 · Human MSCs were incubated with antibiotics-free CCM in twenty 150 cm 2 cell culture plates and transfected directly with miR-146a-5p inhibitor or miRNA scramble control using riboFECTTM CP Transfection Kit for 48 h after the cell density reached about 50% confluency. WebbRiboFECTTM CP reagent for 24 hours accord-ing to the manufacturer’s protocol, followed by reperfusion as described above. 4. MiR-17 inhibitor group cells were transfected with 50.0 nM miR-17 inhibitor or miR-NC, fol-lowed by reperfusion. 5. Stat3 small interfering RNA (siRNA) group cells were treated with Stat3 siRNA (50-CCACGUU
Webb24 jan. 2024 · AML12 cells were transfected with miR-101 mimetics or miR-101 inhibitors using RiboFECTTM for 48 h at 37°C, according to the reagent manufacturer's protocol. Following differentiation and treatments, cells were fixed with 4% paraformaldehyde for 15 min at room temperature and permeabilized with 0.1% Triton X-100 for 5 min at room … Webb18 nov. 2024 · The siRNAs were transfected with riboFECTTM CP Reagent according to the manufacturer’s protocol. Bronchial epithelial cells WT mice were transiently transfected with 20 nM scramble or siRNA. After transfection for 24 h at 37 °C in 5% CO 2 , the cells were prepared as IL-17A group, CSE group, CSE + IL-17A group and controls as …
Webb公司简介： 广州市锐博生物科技有限公司（简称“锐博生物”）由国家千人计划特聘专家和诺贝尔生理/医学奖获得者领衔于 ...
WebbLong RNA Transfection. Cell transfection is one of the important methods for foreign genes entering into eukaryotic cells. Those foreign DNAs or RNAs move into cells by the ancillary packaging of transfection reagents, play its role to further study its cell functions and corresponding influences. mRNA transfection does not need to enter into ... bogdanovich summer campWebb20 apr. 2024 · According to the manufacturer’s protocol of transfection reagent, the miRNAs was diluted with riboFECTTM CP buffer at 37°C for 10 min. The diluent was mixed with riboFECTTM CP Reagent and incubated for 10 min at 37°C. Then, the riboFECTTMCP-miRNA mixture was added to the cells together with 1.8 ml DMEM and incubated at 37°C … global x s\u0026p 500 catholic values etf cathWebb29 jan. 2024 · Transfection reagent riboFECTTM CP and siRNAs that specifically inhibit GPX4 were provided by Ribobio (Guangzhou, China). Animals and experimental protocol. bogdanovich recreation center